The effect of human SOD on the survival rate in rats with temporary splanchnic ischemia

The accumulation of oxygen free radicals is reported to occur in the organs subjected to temporary ischemia followed by reperfusion, resulting in the fatal outcome of the animals. The effects of human SOD, a representative scavenger of oxygen free radicals, on the survival rates were investigated in the rats with temporary splanchnic ischemia. The temporary ischemia was induced by the occlusion of anterior mesenteric and celiac arteries for 30min under anesthesia. Prior and after treatment with 2mg/100g of human SOD, iv or sc, produced significant improvements in survival rates. Human SOD, cloned from human placenta DNA and expressed in microorganisms, has extreme homogeneity. The results suggest the possible introduction of human SOD into clinical field as an effective scavenger of oxygen free radicals.(Ogawa R, Bitoh H, Ohi Y: The effect of human SOD on the survival rate in rats with temporary splanchnic ischemia. J Anesth 2: 41–45, 1988)     

A reevaluation of the status of cholesterol in erythrocytes infected by Plasmodium knowlesi and P. falciparum

The cholesterol synthesis of rhesus monkey erythrocytes parasitized by Plasmodium knowlesi and human erythrocytes infected by P. falciparum, as measured by incorporation of [1-¹⁴C]acetate and ³H₂O, was almost undetectable, concordant with very low levels of measurable 3-hydroxy-3-methyl glutaryl-CoA reductase activity. In addition, both types of infected cells exchanged cholesterol with the plasma at the same rate as uninfected cells. The data do not exclude the possibility of cholesterol transfer from uninfected to infected cells.     

老年冠心病患者血浆HCY、CRP和血脂水平变化及其与冠脉病变程度的关系

目的:探讨冠心病(CHD)患者血浆同型半胱氨酸(HCY)水平与冠脉病变程度及C-反应蛋白(CRP)、血脂水平的关系。方法:检测102例CHD患者和27例正常对照人群的血浆HCY和CRP、甘油三酯(TG)、总胆固醇(TC)和脂蛋白(a)[LP(a)]水平,并进行比较及相关性分析。结果:CHD组血浆HCY以及CRP、TG、TC、LP(a)水平显著高于正常对照组;CHD组血浆HCY水平与CRP、TG、TC、LP(a)无明显相关性(P>0.05),血浆HCY、CRP、TG、TC、LP(a)水平随着冠脉病变支数增加而增高,呈显著正相关。结论:高血浆HCY是CHD的独立危险因素,对CHD的预防和诊断具有一定的临床意义。     

超氧自由基和血液粘度的相关性研究

本文对69名体检人员进行过氧化脂质(LPO)血液流变性和血脂检测,应用电子计算机和医用统计软件进行数据分析.直线回归分析表明,LPO与全血和血浆粘度无显著相关;进行多元逐步回归分析表明,LPO在全血粘度比值中则列为第3受选因子,说明LPO对血浆粘度有一定影响.     

757例体检人群血脂水平调查分析

目的 :了解“健康”体检人群不同年龄组的血脂水平。方法 :对75 7例体检人群的血清甘油三酯 (TG)、总胆固醇 (TC)、高密度脂蛋白胆固醇 (HDL C) ,按年龄分组 ,采用SPSS10 .0软件进行统计分析。结果 :2 0～ 2 9岁组的TG (1.2 8± 0 .63mmol/L)与 3 0～ 3 9岁组(1.5 6± 0 .78mmol/L)和 40～ 49岁组 (1.5 1± 0 .79mmol/L)均有显著性差异 (P <0 .0 1) ;其它各组间无显著性差异。 2 0～ 2 9岁组的TC (4 .5 8± 0 .88mmol/L)与 3 0～ 3 9岁组 (4 .97± 0 .97mmol/L)、40～ 49岁组 (5 .0 0± 1.0 3mmol/L)、5 0～ 5 9岁组 (5 .0 0± 1.0 4mmol/L)、60～ 69岁组 (5 .0 7± 0 .96mmol/L)均有显著性差异 (P <0 .0 1);其它各组间无显著性差异。HDL C水平 70～ 77岁组均显著高于其它各年龄组 ,而其它各年龄组间无统计学差异。TG ,TC ,HDL C的异常率分布在各年龄组间无统计学差异 (P >0 .0 5 )。结论 :75 7例体检人群血脂水平呈升高趋势 ,中青年人群更为明显 ,应当提倡文明饮食和健康的生活方式 ,加强健康教育     

Effect of a new inhibitor of lipid peroxidation on kidney function after ischaemia-reperfusion. A study on rat and rabbit kidneys

Lipid peroxidation of mitochondrial and cell membrane structures is the final step in the oxygen radical-induced damage observed at reperfusion of kidneys after ischaemia. We compared the ability of an indeno-indol compound (code name H290/51) with that of α-tocopherol to inhibit lipid peroxidation in reoxygenated isolated rat renal tissue in vitro measured as production of TBARS (thiobarbituric acid reactive substances). H290/51 was 100 times more efficient than α-tocopherol. Treatment of rats in vivo with H290/51 in a dosage giving a plasma concentration of 500 nmol L^-1 inhibited TBARS production measured in vitro by 80%. Treatment of rabbits with H290/51 almost completely inhibited radical production at reperfusion after 60 min of ischaemia measured with spin trap technique using OXANOH (2-ethyl-3-hydroxy-2,4,4-trimethyloxazolidine) as a spin trap. Furthermore, such pretreatment significantly improved kidney function and survival of rabbits subjected to 60 min of ischaemia to the left kidney and contralateral nephrectomy. These studies stress the importance of inhibiting lipid peroxidation to prevent the ischaemia-reperfusion damage and furthermore suggest a role for treatment with antioxidants like H290/51 in clinical practice, e.g. at reconstructive renal surgery and transplantation.     

中华眼镜蛇毒组分H对实验性动脉粥样硬化的影响

本实验在新西兰兔食饵性动脉粥样硬化模型上,观察了中华眼镜蛇毒组分H预防性给药和治疗性给药对血清胆固醇、过氧化脂质及一氧化氮的影响。发现组分H(2mg·D-1)与2%胆固醇同时喂服或喂胆固醇4周后以组分H(4mg·D-1)治疗数周,均能有效抑制高脂所致的一氧化氮降低和过氧化脂质升高,并能保护超氧化物歧化酶活性和降低血清总胆固醇及甘油三酯的水平。     

Studies on fine structure and location of lipids in quick-freeze replicas of atherosclerotic aorta of WHHL rabbits

Summary The fine structure of intracellular and extracellular lipids in the atherosclerotic aorta of Watanabe-heritable hyperlipidemic (WHHL) rabbits was demonstrated by a quick-freeze etching technique. Many lipid droplets, with and without a membrane, were observed in the foam cells. Membrane-free droplets were observed as onionlike structure with a concentric lamellar structure surrounded by 10 nm filaments. Droplets surrounded by a limited membrane probably correspond to lipid-laden lysosomes.In the extracellular connective tissue space, marked accumulation of lipids with a vesicular structure was seen among collagen fibers. The appearance of these lipids was similar to that of lipids in lysosomes of foam cells.